English translation for "gel fraction"
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- 胶凝部分
Related Translations:
gel: n.冻胶，凝胶(体)；定型发胶。vi.(-ll-) 成冻胶，胶化。
- Example Sentences:
| 1. | A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain , was selected to study as target gene . referred to the reported sequence , rwo primers were designed and synthesized . hn gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method
为此，从澳大利亚某大学兽医病理系引进一株ndv热稳定性天然弱毒b95株，并选取其表面抗原血凝素?神经氨酸酶的编码基因为目的基因，根据国外已发表的序列，设计一对引物，利用rt - pcr技术扩增出了ndvb95株hn基因。 | | 2. | Major difference of hn proteins lies in no . 18 - no . 75 amino acid residues on n - terminal which includes three active sites of hn protein . the influence on biological action , caused by the difference of hn protein , is needed to study further . hn gene has only four glycosylation sites , which is 1 or 2 less on amount than that of other strains . so , this difference can be take on as a natural mark of ndv b95 strain furthermore , the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme , and purified by agrose gel fraction method . then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者将正确的重组克隆质粒pgem - hn用saii 、 saci双酶切，电泳回收目的片段，将其亚克隆到经saii 、 saci双酶切处理的pet - 28c中，转化大肠杆菌tgi感受态细胞，得到的转化子经pcr鉴定和酶切分析，筛选出符合阅读框的重组子，构建成重组表达质粒pet - hn ，并在大肠杆菌bl _ ( 21 ) ( de _ 3 )宿主菌中成功地表达了含目的蛋白的融合蛋白，融合蛋白的分子量约66kd ，加入iptg诱导8h后，蛋白表达几乎达到最高水平。 |
- Similar Words：
- "gel for external use" English translation, "gel for manufacturing" English translation, "gel formation" English translation, "gel forming" English translation, "gel forming agent" English translation, "gel fractionator" English translation, "gel free" English translation, "gel general emulation language" English translation, "gel grain sugar" English translation, "gel growth" English translation
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